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rqmicro.COUNT

Portable cartridge-based flow cytometer for quantitative analysis of bacteria in liquid samples
viable but non-culturable cells (VBNC)

When every cell counts, cultivation puts you at risk

Cultivation on agar plates comes with serious limitations. It fails to detect a significant portion of viable microorganisms. Many bacteria are viable but non-culturable (VBNC), meaning they are alive and potentially harmful but do not grow into visible colonies on agar. This blind spot is not only critical in routine testing for total viable cells but also for pathogens like Legionella and E. coli.

As long as testing relies solely on cultivation, there is a substantial risk of underestimating microbial contamination, putting your operations, public health and safety at risk. To ensure accurate results and true risk assessment, it's essential to adopt a method to directly quantify all viable cells, not just those that grow on plates.

In mineral water, up to 99% of viable cells are VBNC - meaning only 1% are detected by traditional cultivation methods. Users of the gold standard are basing their decisions on just a tiny fraction of the actual microbial load. Read more about Quantifying Viable Cells and VBNC and food safety

Only a fraction of Legionella cells will grow on agar, even after 10 days of incubation. Water treatment does typically reduce the fraction of Legionella that grow on agar, but the danger remains. Read more the Inaccuracies of the ISO 11731 method in this scientific study or in our blog about Legionella detection with flow cytometry.

The dangerous E. coli O157 strain is generally missed by the gold-standard test because this subtype does not produce beta-glucuronidase, an enzyme commonly targeted in culture-based detection. Read more in this blog post.

See more. Sooner.

Single-cell analysis on rqmicro.COUNT, powered by flow cytometry technology, enables users to optically quantify cells in liquid samples including cell viability detection. This cultivation-independent method includes viable but non-culturable cells (VBNC) and is available for total bacteria as well as for specific cells including Legionella and E. coli

Accurate and reliable results, available within short time on rqmicro.COUNT and the Cloud Solution, provide the required data for managing water recirculation systems, in quality control, in biopharma, in Life Sciences research and more.

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Quantitative analysis of specific pathogens and total bacteria
A user-friendly cartridge-based flow-cytometer

Automated workflow for unbiased results in labs and on-site

rqmicro.COUNT provides a complete solution based on single-use cartridges. The automated workflow includes a flow cytometric quantification of cells from various water types optionally combined with a fast immunomagnetic separation of specific target cells. 

The cartridge system enables a highly efficient and controlled process, eliminates sample-to-sample contamination, and does not require lengthy start-up or maintenance cycles.

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rqmicro Cloud Solution

Cloud Solution

Results generated by rqmicro.COUNT are immediately available online on the Cloud Solution.


Cloud Solution features include:

  • Monitoring critical control points
  • Receipt of alerts
  • Creation of reports
  • Sharing results

The Cloud Solutions enables effective water microbiology risk management with different stakeholders.


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Online platform for reports and data analysis

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Want to know more about the rapid pathogen detection with rqmicro.COUNT? Contact us or book an online demo presentation to see rqmicro.COUNT in action. 




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rqmicro.COUNT Specifications

ANALYTICAL SYSTEM

Measurement technology: Fluorescence-based flow cytometric analysis

Cell sorting capability: Immunomagnetic separation (IMS)

Excitation: 1 Laser at 488nm wavelength

Detection: 2 fluorescence channels, up to 10‘000 events per second

Sample volume: 200 μL up to 1 mL

Flow rate: 60 μL / min (during measurement)

USER INTERACTION & CONNECTIVITY

User interface: 10 inch capacitive LCD touch screen

Language support: English, German, Chinese

Connectivity: 2x USB, 1x Ethernet, USB dongle ready for WLAN/cellular networks

Software upgrades: Online delivery or via USB flash drive

OPERATING CONDITIONS & DIMENSIONS

Power consumption: 60 W, 12 V DC, 7.5 A , AC benchtop adapter, 100 - 240 V, 50/60 Hz

Operating environment: Temperature: 15 °C – 27 °C, non-condensing

Physical dimensions: B x W x H (cm): 22.5 x 23.5 x 46.5, Weight: 12.9 kg

Safety compliance: EN 61010-1

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