Technology

Building blocks of the rqmicro solutions

Integrated Technology Domains

rqmicro.COUNT is built on the integration of four fundamental technology domains.
Each domain contributes a defined and necessary function within the analytical system.

Immunoassays provide specificity, flow cytometry performs quantitative single-cell measurements, the single-use cartridge automates the full analytical workflow, and software coordinates the entire system. Together they form a unified platform for rapid microbiological analysis that can be continuously expanded through new assays and computational methods.

Immunoassays

Immunoassays provide the biological specificity of the rqmicro.COUNT platform.

rqmicro develops and optimizes assay chemistry, fluorescent labeling strategies and reagent formulations for reliable detection performance in complex sample matrices.

rqmicro uses immunomagnetic separation, also supported by the rqmicro.COUNT instrument, to automatically isolate target cells before flow cytometry quantification.

Immunoassays are provided in stable, lyophilized formulations, pre-filled in single-use tubes.

Flow Cytometry

Flow cytometry is the analytical engine of rqmicro.COUNT, providing rapid quantitative analysis of individual microorganisms.

The complete measurement chain from laser optics to fluorescence detection and signal processing is optimized as an integrated system. High-throughput single-cell analysis delivers statistically robust results within minutes. Multiparametric measurements enable sensitive discrimination of target organisms from complex sample backgrounds. This measurement platform provides the flexibility to support a growing portfolio of microbiological assays.

Single-Use Cartridge Technology

The analytical workflow is implemented on a disposable cartridge. A revolution in flow cytometry.

The result is a robust solution which structurally isolates samples in single-use consumables, thereby eliminating carry-over risk from sample to sample and all regular cleaning and maintenance cycles which classical flow cytometry is prone to.

Software & Computational Methods

High-performance computational methods transform optical measurements into quantitative microbiological information.

The software architecture enables application-specific optimization of analytical performance without modifications to the hardware platform. Standardized workflows, traceability and controlled user interaction support reproducible laboratory operation and quality management requirements.