A unique combination of three technologies lies at the heart of rqmicro‘s rapid test method:
Automated water analysis
rqmicro instruments isolate target cells via magnetic particles that are coupled to specific antibodies. In contrast to conventional IMS methods, our automated approach separates target cells contact-free, gentle and on a single-use microfluidic cartridge.
Sample resuspension into the cartridge after a standard water filtration step.
rqmicro reagents bind to target cells and thereby enable the automated immunomagnetic separation. Another set of reagents enables fluorescence detection on rqmicro instruments or third-party flow cytometers.
Capturing of target cells during automated sample processing and isolation of target cells in a positive fraction for flow cytometric analysis.
rqmicro uses the great potential of microfluidics to deliver a product that allows for a highly efficient separation and purification of target cells from samples of varying complexity. As the sample passes through a narrow channel in the microfluidic cartridge, target cells are isolated and resuspended in clean buffer solution. This positive fraction can be analyzed by flow cytometry or any method of analysis by your choice.
Resuspension of the sample onto a single-use cartridge together with the washing buffer.
Automated and effective purification of the target cells on the cartridge using external sample actuation.
Transition of the purified and concentrated target cells into the positive fraction for downstream analysis by flow cytometry.
Flow Cytometric Detection
rqmicro products are designed to empower your in-house flow cytometer to detect specific pathogens. Since it is a cultivation-independent method, all fluorescence labelled cells present in a sample, also viable but non-culturable (VBNC) cells, are quantified by FCM in a matter of minutes.
Flow cytometry is a laser-based technology for electronic cell counting that allows multiparametric analysis of the properties of thousands of cells per second. The cells labelled with a fluorescent dye pass in front of a laser in a stream of fluid. The laser excites electrons in the fluorescent dye that then return in fractions of a second to their previous state by emitting light. The FCM detects this light signal, which is then transformed into data that is analyzed by a computer using special software.
After immunomagnetic separation, the positive fraction is transferred to fresh tubes. At this point, if a viability assessment is of interest, half of the sample can be transferred into a fresh tube and the rqmicro viability dye can be added.
Then the sample is ready to be analyzed with your flow cytometer. Depending on the instrument, the samples can be analyzed in tube-mode or also in 96-well-plates.
The total and viable cell count of the target cells can then be obtained in a matter of minutes. The cell population is usually visualized in a dot plot.