Legionella pneumophila Kits
Quantify Legionella in less than 2 hours
rqmicro developed these highly specific kits to simplify, accelerate and improve the detection of Legionella pneumophila in aqueous water samples from tap water to samples with highly complex matrices such as cooling tower water. The L. pneumophila Kits present a revolutionary solution for Legionella analysis and thus open a new chapter in the field of pathogen detection and in the monitoring of water quality.
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Speed
- Time to result less than 2 hours
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Specificity
- High specificity and sensitivity
- Detection of viable but non-culturable cells
- Discrimination of dead cells
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Application
- Aqueous samples
- Compatible with flow cytometry, cultivation, PCR, microscopy, etc.
Video Demonstration
The kits from rqmicro are available in different versions. According to the separation instrument and analysis method of your choice, our product range offers the optimal solution for you.
Organism |
Quantification Step |
Purification Step* |
Kit Name |
| L. pneumophila | Flow Cytometry | CellStream | L.p. SG1-14 DETECT Kit for the CellStream |
| MIMS | L.p. SG1-14 DETECT Kit for the MIMS | ||
| Cultivation | CellStream | L.p. SG1-15 SEP Kit for the CellStream | |
| MIMS | L.p. SG1-15 SEP Kit for the MIMS |
*CellStream: automated IMS; MIMS: manual IMS
All kits are available in a PLUS option to meet the increased requirements for the precise and reliable analysis of complex aqueous matrices, e.g. process or cooling tower water.
Legionella pneumophila SG1-14 Detection Kits (Early Release)
Separation and flow cytometric detection
The perfect solution for ultrafast and precise analysis of Legionella pneumophila SG1-14 with a standard flow cytometer including VBNC detection and viability assessment.
| L.p. SG1-14 DETECT Kit (96 Tests) for the CellStream |
| L.p. SG1-14 DETECT PLUS Kit (96 Tests) for the CellStream |
| L.p. SG1-14 DETECT Kit (96 Tests) for the MIMS |
| L.p. SG1-14 DETECT PLUS Kit (96 Tests) for the MIMS |
Less than 2 hours (60 min incubation time)
DETECT Kit: Tap water
DETECT PLUS Kit: Industrial water and environmental water
| Magnetic particles: | Antibody-coated magnetic particles |
| Staining dye: | Labelling antibody |
| Viability dye: | Propidium iodide as an indicator of membrane integrity |
| Positive control: | Lyophilized, chemically fixed Legionella pneumophila SG1-cells (Philadelphia) |
| Filters: | Polycarbonate filters; pore size 0.22 µm; Ø 47 mm |
| Buffer 1: | Incubation buffer |
| Buffer 2: | Separation buffer |
| Filters for pre-filtration (for all PLUS Kits): | Polycarbonate filters; pore size 5 µm |
| Cartridges (for CellStream DETECT/DETECT PLUS Kits): | Disposable microfluidic cartridges for the CellStream |
| Tubes (for MIMS DETECT/DETECT PLUS Kits): | 5 mL tubes |
CellStream – for automated sample preparation
MIMS – for manual sample preparation
Legionella pneumophila SG1-15 Separation Kits (Early Release)
Separation and purification of target cells for cultivation and other types of analysis
The separation kit efficiently isolates L.p. SG1-15 target cells and at the same time eliminates more than 95% of the competing flora. It thus enables reliable and conclusive results when cultivating cells on agar or using other methods of downstream analysis.
- Tailored to complex matrices such as industrial cooling tower or environmental water samples
- Elimination of 95% – 99% of the competing flora
- No potentially detrimental acid or heat treatment needed
- Reliable results on agar plates
| L.p. SG1-15 SEP Kit (96 Tests) for the CellStream |
| L.p. SG1-15 SEP PLUS Kit (96 Tests) fort the CellStream |
| L.p. SG1-15 SEP Kit (96 Tests) for the MIMS |
| L.p. SG1-15 SEP PLUS Kit (96 Tests) for the MIMS |
Separation and purification in less than 2 hours (60 min incubation)
SEP Kit: tap water
SEP PLUS Kit: industrial water, environmental water
| Magnetic particles: | Antibody-coated magnetic particles |
| Filters: | Polycarbonate filters; pore size 0.22 µm; Ø 47 mm |
| Buffer 1: | Incubation buffer |
| Buffer 2: | Separation buffer |
| Filters for pre-filtration (for all PLUS Kits): | Polycarbonate filters; pore size 5 µm |
| Cartridges (for CellStream SEP/SEP PLUS): | Disposable microfluidic cartridges |
| Tubes (for MIMS SEP/SEP PLUS): | 5 mL tubes |
CellStream – for automated sample preparation
MIMS – for manual sample preparation