Legionella pneumophila SG1 Kits

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Quantify Legionella in less than 2 hours

rqmicro developed these highly specific kits to simplify, improve and accelerate the detection of Legionella pneumophila SG1. The L.p. SG1 Kits present a revolutionary solution for Legionella analysis and thus open a new chapter in the field of pathogen detection and in the monitoring of water quality.

  • Speed

    • Time to result less than 2 hours
  • Specificity

    • High specificity and sensitivity
    • Detection of viable but non-culturable cells
    • Discrimination of dead cells
  • Application

    • Aqueous samples
    • Compatible with flow cytometry, cultivation, PCR, microscopy, etc.

Video Demonstration

The kits from rqmicro are available in different versions. According to the separation instrument and analysis method of your choice, our product range offers the optimal solution for you.

Organism

Quantification Step

Purification Step*

Kit Name

Legionella pneumophila SG1 Flow Cytometry CellStream L.p. SG1 DETECT Kit for the CellStream
MIMS L.p. SG1 DETECT Kit for the MIMS
Cultivation CellStream L.p. SG1 SEP Kit for the CellStream
MIMS L.p. SG1 SEP Kit for the MIMS

*CellStream: automated IMS; MIMS: manual IMS

All kits are available in a PLUS option to meet the increased requirements for the precise and reliable analysis of complex aqueous matrices, e.g. process or cooling tower water.

Legionella pneumophila SG1 Detection Kits

Separation and flow cytometric detection

The perfect solution for ultrafast and precise analysis of Legionella pneumophila SG1 with a standard flow cytometer including VBNC detection and viability assessment.

L.p. SG1 DETECT Kit (96 Tests) for the CellStream
L.p. SG1 DETECT PLUS Kit (96 Tests) for the CellStream
L.p. SG1 DETECT Kit (96 Tests) for the MIMS
L.p. SG1 DETECT PLUS Kit (96 Tests) for the MIMS

Less than 2 hours (30 min incubation time)

DETECT Kits: Tap water
DETECT PLUS Kits: Industrial water and environmental water

Magnetic particlesAntibody-coated magnetic particles
Staining dyeLabelling antibody
Viability dyePropidium iodide as an indicator of membrane integrity
Positive controlLyophilized, chemically fixed
Legionella pneumophila SG1-cells (Philadelphia)
FiltersPolycarbonate filters; pore size 0.22 μm; Ø 47 mm
Buffer 1Incubation buffer
Buffer 2Separation buffer
Cartridges (for CellStream DETECT/DETECT PLUS Kits)Disposable microfluidic cartridges
Tubes (for MIMS DETECT/DETECT PLUS Kits)5 mL tubes
Filters for pre-filtration (for all DETECT PLUS Kits)Polycarbonate filters; pore size 5 μm

CellStream – for automated sample preparation

MIMS – for manual sample preparation

Performance

Sample purity:                 >95%*

Limit of detection:            20 – 50 cells / liter*

Time to result:                 Less than 2 hours

*Dependent on FCM instrument and sample matrix

The sensitivity of the rqmicro method is 10 – 40 fold higher compared to ISO 11731 (n ≥ 3, bars SD). Results were obtained under laboratory conditions. Depending on the sample type, this value may change.

Real time monitoring of a disinfection process at 70 °C

Viable but non-culturable (VBNC) bacteria refer to bacteria that are in a state of low metabolic activity. They do not divide, but are alive and have the ability to regain infectivity once resuscitated.

  • The rqmicro method reveals that after a disinfection process viable Legionella cells are potentially still present after 60 minutes.
  • The cultivation method does not detect VBNC bacteria as VBNC cells are unable to grow on agar.

Cross reactivity experiments with the rqmicro® Legionella pneumophila SG1 Kit using various SG1 strains portray high specificity, a complete coverage of SG1 and no cross reactivity with other bacterial cells. Additional cross reactivity experiments are ongoing.

Organism L.p. SG1Antibody binding
Legionella pneumophila PhiladelphiaDSM7513+
Legionella pneumophila AllentownATCC43106+
Legionella pneumophila BellinghamDSM25214+
Legionella pneumophila BenidormDSM25199+
Legionella pneumophila BenidormDSM27564+
Legionella pneumophila Cambridge-1NCTC11231+
Legionella pneumophila CamperdownATCC43113+
Legionella pneumophila FranceATCC43112+
Legionella pneumophila HeyshamATCC43107+
Legionella pneumophila KnoxvilleDSM25070+
Legionella pneumophila OLDADSM25200+
Legionella pneumophila OxfordDSM25213+
Legionella pneumophila Philadelphia-2NCTC11193+
Legionella pneumophila PontiacNCTC11191+
Legionella pneumophila WashingtonNCTC11201+
Legionella anisaLegionella micdadeiCampylobacter jejuni
Legionella birminghamensisLegionella oakridgiensisEscherichia coli
Legionella bozemaniiLegionella parisiensisSalmonella typhimurium
Legionella cincinnatiensisLegionella rubilucensCitrobacteri gillenii
Legionella dumoffiiLegionella taurinensisListeria monocytogenes
Legionella jamestowniensisPseudomonas aeruginosaListeria welshimeri
Legionella jordanisPseudomonas fluorescensListeria fleishmanii
Legionella londoniensisStaphylococcus aureus
Legionella longbeachaeStaphylococcus epidermidis

Legionella pneumophila SG1 Separation Kits

Separation and purification of target cells for cultivation and other types of analysis

The separation kit efficiently isolates Legionella pneumophila SG1 target cells and at the same time eliminates more than 95% of the competing flora. It thus enables reliable and conclusive results when cultivating cells on agar or using other methods of downstream analysis.

L.p. SG1 SEP Kit (96 Tests) for the CellStream
L.p. SG1 SEP PLUS Kit (96 Tests) for the CellStream
L.p. SG1 SEP Kit (96 Tests) for the MIMS
L.p. SG1 SEP PLUS Kit (96 Tests) for the MIMS

Purification of target cells in less than 2 hours (30 min incubation).

SEP Kit: Tap water
SEP PLUS Kit: Industrial water and environmental water

Magnetic particlesAntibody-coated magnetic particles
FiltersPolycarbonate filters; pore size 0.22 μm; Ø 47 mm
Buffer 1Incubation buffer
Buffer 2Separation buffer
Cartridges (for CellStream SEP/SEP PLUS Kits)Disposable microfluidic cartridge
Tubes (for MIMS SEP/SEP PLUS Kits)5 mL tubes
Filters for pre-filtration (for all PLUS Kits)Polycarbonate filters; pore size 5 μm

CellStream – for automated sample preparation

MIMS – for manual sample preparation